40.7% increase in Immunoglobulin A (IgA) level after 6 weeks.

A double-blind, placebo-controlled study conducted with Texas A&M evaluated shifts in Immunoglobulin A (IgA) levels in 26 healthy adult dogs (ages 1.5 to 4.5 years) over six weeks. The study compared E4L—a synbiotic blend of pre-/probiotics and bovine colostrum—to a base placebo powder, using the ELISA (Enzyme-linked Immunosorbent Assay) test to measure Monomeric, Dimeric, and Secretory IgA levels. Stool samples were collected at baseline, three weeks, and six weeks to assess changes in IgA concentrations.

Why This Matters: Elevated IgA levels play a vital role in building immune defenses, supporting resilience against environmental stressors, and supporting seasonal allergy responses

70.7% increase in beneficial bacteria compared to placebo after 3 weeks.

A double-blind, placebo-controlled study conducted with Texas A&M evaluated the effects of E4L’s pre-/probiotic blend on the gut microbiome and beneficial bacterial growth in dogs. Over three weeks, 26 healthy adult dogs were randomly assigned to receive either E4L or a placebo. Fecal samples collected before and after the trial were analyzed using qPCR to assess microbiome changes and the Bristol Stool Chart to evaluate stool consistency.

Why this matters: Beneficial bacteria improve dog health by aiding digestion, producing nutrients, and strengthening the gut lining. They boost the immune system, reduce inflammation, and help prevent diarrhea and gut problems. Overall, they support better digestion, immunity, and long-term health.

19.6% better stool scores

An observational trial evaluated the effects of E4L’s proprietary prebiotic/probiotic blend on the gut microbiome and stool consistency of adult dogs, using the Bristol Stool Scale. Over three weeks, randomized adult dogs were randomly assigned to receive either E4L or a placebo (13 per group).

Why this matters: better poop score (ideal Bristol type 2–3: firm, well-formed, easy to pick up) shows healthy digestion, good nutrient absorption, and proper gut function. It reflects a balanced microbiome and healthy gut lining. Improving poop scores helps reduce diarrhea, constipation, and inflammation while serving as a simple daily indicator of your dog’s overall gut health.

143.9% improvement in dysbiosis index:

A double-blind, placebo-controlled study conducted with Texas A&M evaluated the effects of E4L’s synbiotic blend of pre-/probiotics, bovine colostrum, protein, and omega-3s on the gut microbiome of healthy adult dogs. Improved dysibiosis scores are connected to gut stability and whole body immune function

Why this matters: Dysbiosis Index improvement = a healthier, more balanced gut. When the score goes down, it means good bacteria are winning, harmful bacteria are decreasing, and the gut is functioning better—leading to stronger immunity, better stool, and overall health.