Our formulas are driven by real clinical research
Clinical research is at the heart of our mission to move dog supplements away from flashy marketing with no substance. Our Proven-First mission focuses on clinical-based trials to demonstrate formula effectiveness.
40.7%
increase in Immunoglobulin A (IgA) level after 6 weeks.
Clinical
A double-blind, placebo-controlled study conducted with Texas A&M evaluated shifts in Immunoglobulin A (IgA) levels in 26 healthy adult dogs (ages 1.5 to 4.5 years) over six weeks. The study compared E4L—a synbiotic blend of pre-/probiotics and bovine colostrum—to a base placebo powder, using the ELISA (Enzyme-linked Immunosorbent Assay) test to measure Monomeric, Dimeric, and Secretory IgA levels. Stool samples were collected at baseline, three weeks, and six weeks to assess changes in IgA concentrations.
Key result: Data suggests that E4L’s pre-/probiotic properties combined with bovine colostrum help boost our dog’s immune function.
Why it matters
Higher IgA levels strengthen gut immunity, helping dogs fight off bacteria, viruses, and toxins while reducing inflammation.
1. Compared to dogs not receiving E4L
70.7%
increase in beneficial bacteria compared to placebo after 3 weeks.
Clinical
A double-blind, placebo-controlled study conducted with Texas A&M evaluated the effects of E4L’s pre-/probiotic blend on the gut microbiome and beneficial bacterial growth in dogs. Over three weeks, 26 healthy adult dogs were randomly assigned to receive either E4L or a placebo. Fecal samples collected before and after the trial were analyzed using qPCR to assess microbiome changes and the Bristol Stool Chart to evaluate stool consistency.
Comparison: Dogs taking E4L showed enrichment in all four bacteria strains. Where beneficial bacteria increased by +1.47 log dna after 3 weeks.
Why it matters
Beneficial bacteria support a balanced gut biome, which plays a key role in digestion, immunity, and overall health.
1. Compared to dogs not receiving E4L
143.9%
improvement in Dysbiosis Index score after 3 weeks.
Clinical
A double-blind, placebo-controlled study conducted with Texas A&M evaluated the effects of E4L’s synbiotic blend of pre-/probiotics, bovine colostrum, protein, and omega-3s on the gut microbiome of healthy adult dogs. Using the Dysbiosis Index (DI), a qPCR-based assay that quantifies eight key bacterial groups in fecal samples, the study measured changes in microbial diversity and gut health. 26 dogs were randomly assigned to receive either E4L or a placebo for three weeks, with fecal samples collected before and after the trial to assess shifts in microbiome composition and bacterial activity.
Comparison: Dogs taking E4L saw 32.9% greater improvement in DI scores compared to placebo.
Why it matters
A diverse, healthy microbiome impacts everything from digestion and inflammation to allergies and skin or coat health.
1. Compared to dogs not receiving E4L
19.6%
better fecal score on the Bristol Stool Scale after 3 weeks.
Observational
An observational trial evaluated the effects of E4L’s proprietary prebiotic/probiotic blend on the gut microbiome and stool consistency of adult dogs, using the Bristol Stool Scale. Over three weeks, 26 healthy dogs were randomly assigned to receive either E4L or a placebo (13 per group). Fecal samples collected before and after supplementation were analyzed to assess changes in stool characteristics and the digestive benefits of daily E4L use.
Comparison: Dogs taking E4L improved their poo score 15.4% more than those given placebo – demonstrating firmer, well formed stool.
Why it matters
Firm, well-formed poops reflect good digestion and nutrient absorption—key signs of better energy and wellness.
1. Compared to dogs not receiving E4L
Third-Party Certification
Our approach to certifications shows you that someone else is looking under the hood. Safe, transparent ingredients start with third-party validation during the sourcing and manufacturing process. E4L products are regulated and guided by the following organizations.
NSF Certified
NSF is one of the most rigorous independent quality and safety certification programs in the supplement industry.
NASC Membership
The National Animal Supplement Council is an organization dedicated to improving the quality of supplements in companion animals.
Human Grade – GMP 21 CFR Part 117 Certified
Every ingredient and the resulting product is stored, handled, processed and transported consistent with human grade regulat.
What people are saying
We started our girl Lady out on E4L and absolutely loved the results! It really complements her current diet. Loved that the ingredients were clinically backed and devoted to gut biome and immune support. Bravo!
Nutrition is the utmost important for our two bulldogs Henry & Beatrice. E4L has been an amazing addition to our dog’s diet. Love the Omega-3s which are very important to us and that it has Pre AND Probiotics for their belly health. Having Senior bulldogs I look for products that have no synthetics and love that E4L is human grade!
My dog enjoys the flavor of this supplement, it wasn’t hard to get him to eat it! I noticed an increase in energy levels. I also used this supplement during the time my dog was healing from a broken leg; I believe it sped up the healing process and decreased inflammation.
Great product! I had never used a supplement for my dog before and I was quite pleased with how much more energy and endurance my dog has. She has enjoyed the flavor of E4L and never turned it down when first introduced. Great for closing the gap of nutrition our dog needs for maintaining a healthy and active lifestyle. Looking forward to the long term health benefits it will provide to keep our dog healthy!
This product is AMAZING!! Prior to finding E4L, I was giving my puppy two different types of powders for her daily nutrition but E4L offered even more nutrients in just one powder. Highly recommend this product for all dogs! Her stools have become so much more firm since starting E4L. Not to mention how shiny her coat is! Everywhere we go, people mention how shiny her coat is. This is definitely a product that we will keep her own FOR LIFE!
Our Mochi has always had a bit of sensitive stomach and stools on the softer side. Adding E4L to Mochi’s meals has not only made her movements more regular, but also helped improve her coat and overall health. Would absolutely recommend E4L for other bulldog owners
Absolutely love this product. Not only did my doggo love the taste but lost weight and had more energy then normal. She was happy and full of playtime. Her stool also appeared more healthy.
As someone who works in the veterinary field I’m always looking for the best nutrition supplements for my dogs. E4L provides the added nutrition I’ve been looking for to ensure our dogs are at their healthiest. Our Golden Retriever can be picky but he loves E4L and would finish his bowl every time. Definitely something we will keep using!
In a 30 day observational trial, over 100 dog owners were asked to try E4L and report how their dog responded.*
91%
said E4L closed nutrition gaps in their dog’s diet
79%
said E4L improved their dog’s health & nutritional needs
94.7%
saw increased appetite during mealtime
84%
said E4L was easy to incorporate into their dog’s routine
Data collected include both E4L puppy and E4L adult observational trials.
All participants were asked to use E4L for a 30 day period and report their findings following the trial.
Vet Advisory Panel
E4L’s evidence based approach is led Dr. Mariana and Dr. Brendita who have guided our clinical trial based philosophy in dog nutrition.
Dr. Mariana Lara Duque
MVZ
Founding member of E4L, focused in canine gut biome function efficiency
Dr. Brenda Diaz Lopez
MVZ
Founding member of E4L, interested in neonatal and puppy immune function
